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The structures for the inhibitor bound complexes were retrieved from Protein Data Bank with PDB codes: 1AJX [32] for AHA001 bound complex, 1HVR [33] for XK263 bound complex and 1HXW [34] for ABT538 bound complex.
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Yeast protein complexes were retrieved from CYC2008, an updated archive of Curated Yeast Complexes [7].
However, separated complexes were retrieved as well.
From three patients, 57 cumulus oocyte complexes were retrieved.
Immune complexes were retrieved using anti-Rabbit Ig IP Dynabeads.
Forty-six complexesocyte complexes were retrieved from three patients (mean of 15.3 per patient).
Eighty-one cumulus oocyte complexes were retrieved from seven patients (mean of 11.6 per patient).
Free and bound complexes are resolved on 1.3% agarose gel.
A crystal structure of the inhibitor bound complex is reported.
Each bound complex was simulated twice using different starting velocities.
Cumulus-oocyte complex (COC) were retrieved 36 to 38 h after HCG administration and washed.
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