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The bound complexes were resolved on a 4 12% polyacrylamide gel (Invitrogen), and Coomassie-stained.
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Free and bound complexes are resolved on 1.3% agarose gel.
Binding complexes were resolved by electrophoresis in non-denaturing 6% polyacrylamide gels using 0.5 × TBE as running buffer and assessed by autoradiography.
The structures for the inhibitor bound complexes were retrieved from Protein Data Bank with PDB codes: 1AJX [32] for AHA001 bound complex, 1HVR [33] for XK263 bound complex and 1HXW [34] for ABT538 bound complex.
These complexes were resolved on 10% SDS-PAGE minigels.
Complexes were resolved on 10% Novex Nupage gels (Invitrogen, USA) and transferred to nitrocellulose.
DNA-protein complexes were resolved on 6% polyacrylamide gels in 0.5× TBE buffer.
Complexes were resolved by SDS-PAG and then analyzed by Western blot analysis.
The complexes were resolved by SDS-PAGE under denaturing conditions and Coomasie stained (Fig. 2).
The RNA-protein complexes were resolved on a 5% TBE polyacrylamide gel and visualized by autoradiography.
The ribosome complexes were resolved on sucrose density gradients as described in materials and method.
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