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Images of ligand and receptor bound complexes were prepared in Ligplot visualizing program [ 31] and polar contacts between them were noted down.
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The structures for the inhibitor bound complexes were retrieved from Protein Data Bank with PDB codes: 1AJX [32] for AHA001 bound complex, 1HVR [33] for XK263 bound complex and 1HXW [34] for ABT538 bound complex.
Transfection complexes were prepared in Opti-MEM (Life Technologies).
The DMC complexes were prepared using different complexing and co-complexing agents.
Their lanthanide metal complexes were prepared.
The EVO/HP-β-CD inclusion complexes were prepared successfully.
The miRNA/atelocollagen complexes were prepared immediately before injection.
Immune complexes were prepared using anti-NF-κB p65 antibody.
DNA complexes were prepared at 100 μM as described above.
Transfection complexes were prepared according to the manufacturer's instructions.
Free and bound complexes are resolved on 1.3% agarose gel.
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