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For enhanced loading efficiency, 15 pM of the bound complexes were immobilized onto Magbeads Pacific Biosciences Incc).
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The E1E2 complexes were immobilized on agarose beads bound to a conformation-sensitive monoclonal antibody (mAb H53).
A similar behavior was found also when Mn(Salen Cl complexes were immobilized on those supports.
PBsome complexes were immobilized on the glass slide with polyvinyl alcohol (PVA) solution (solublized with 0.75 M phosphate buffer, pH 7.0).
The resulting cohesin-DNA complexes were immobilized on beads and treated with TEV protease.
In brief, approximately 1000 RU of the biotinylated hCD1d-lipid complexes were immobilized onto streptavidin-coated CM5 sensor chips (Biacore).
A crystal structure of the inhibitor bound complex is reported.
The Flag-CUL1 324-776)/GST-HA-RBX1 complex was immobilized on GSH-sepharose (GE).
As shown in Figure 7F, substantial amount of Alr4641 was bound when NTRC was immobilized on NiNTA agarose.
Recombinant human TLR4-MD2 complexes (R&D Systems, Minneapolis, MN) were immobilized on a CM5 sensor chip at approximately 8000 RU.
Def1 Experiments In Vitro Def1 Binding Experiments Def11-500, Def11-500/CUEm and Dsk2 were immobilized on Affigel-15 (BioRad) as per manufacturers instructions, at 2 mg/ml of resin.
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