Sentence examples for bound complexes were eluted from inspiring English sources

Samples were incubated with Salmon Sperm DNA/Protein A Agarose-50% Slurry and bound complexes were eluted in elution buffer (1% SDS, 0.1 M NaHCO3).

After extensive washing with CB300, bound complexes were eluted with FLAG-peptide and separated by SDS-PAGE.

The lysate was subjected to IgG affinity chromatography and bound complexes were eluted by incubation with AcTEV protease.

Bound complexes were eluted from the beads with elution buffer (10%SDS, 0.1M NaHCO3) followed by heating at 65°C overnight (reverse cross-linking).

Bound complexes were eluted from the beads in elution buffer by heating at 65°C with occasional vortex, and crosslinking was reversed by 6 hr incubation at 65°C.

After several washes with the column buffer, the bound complexes were eluted with 10 µM of streptomycin.

Resulting immune-bound complexes were eluted in Laemmli buffer and submitted to SDS-PAGE and Western blot analysis.

Resin slurries were poured into gravity columns, washed with 0.5 NaCl HGN (0.1% NP-40) with 20 mM imidazole, and bound DKC1 complexes were eluted with buffer 0.3 M NaCl HGN (0.1% NP-40) containing 0.25 M Imidazole.

The antibody/protein/DNA complexes were eluted with 150 ul IP elution buffer (50 mM NaHCO3, 1% SDS) twice.

After incubation, the beads were collected and the DNA-antibody complexes were eluted at 65 °C.

The protein-antibody complexes were eluted followed by detection for STING by immunoblotting.