Sentence examples for bound complexes were detected from inspiring English sources
After washing 4 times, bound complexes were detected according to the protocol of the COMP ELISA.
After washing with PBS 0.05% Tween-20, bound complexes were detected with horseradish peroxidase-conjugated goat anti-mouse IgG (BioRAD, Hercules, CA, USA).
After washing, bound antibody was detected using anti-rabbit antibody linked to horseradish peroxidase and bound complexes were detected using enhanced chemiluminescence.
After washing, bound antibody was detected using anti-rabbit/mouse antibody (1 5000 dilution) linked to HRP, and bound complexes were detected and documented using enhanced chemiluminescence method (Bio-Rad, Hercules, CA, USA) and Geliance imaging system (PerkinElmer, Waltham, MA, USA).
Bound complexes were detected with an Alexa488-labeled anti-penta-HIS mab (Qiagen, Hilden, Germany).
Bound complexes were detected and quantified by scanning densitometry.
After a final washing step, bound antibody complexes were detected by incubation with HRP-substrate ABTS (Roche, Mannheim, Germany) for 20 min at RT in the dark, followed by analysis of the absorption at 405/650 nm in an ELISA reader.
Bound antibodies were detected by using rabbit antibodies against rat IgG that had been conjugated with horseradish peroxidase (HRP) and bound complexes were revealed by staining with tetrametyl-benzidine (KBL, Gaithersburg, MD, USA) and H202.
The structures for the inhibitor bound complexes were retrieved from Protein Data Bank with PDB codes: 1AJX [32] for AHA001 bound complex, 1HVR [33] for XK263 bound complex and 1HXW [34] for ABT538 bound complex.
In the case of endogenous JIP1 also smaller complexes were detected, but they contain bound endogenous VRK2A, which is not detected free (Fig. 8A).
To finally visualize bound primary antibodies, membranes were incubated with horseradish peroxidase-conjugated secondary antibodies and the obtained antibody-containing complexes were detected with the ECL Western blotting detection system (Pierce , Thermo Fischer Scientific Bonn, Germany).
