These biotinylated nucleic acid complexes were incubated with protein lysates and bound complexes were captured on streptavidin magnetic beads and analyzed by immunoblotting.
The structures for the inhibitor bound complexes were retrieved from Protein Data Bank with PDB codes: 1AJX [32] for AHA001 bound complex, 1HVR [33] for XK263 bound complex and 1HXW [34] for ABT538 bound complex.
To determine if this smaller fragment (hereafter SMARCAL1-RBM) bound to RPA32C in the same manner as SMARCAL11 32, a progressive series of complexes were analyzed by 2D N H NMR using [N]RPA32172 270 and [N]RPA32C titrated with SMARCAL11 32 and SMARCAL1-RBM.
Immune complexes were analyzed for Daple and Dvl by IB. (C ) Daple can displace Dvl bound to the cytoplasmic tail of FZD7R in vitro.
Eluted complexes were analyzed by SDS-Page.
Inputs and immune complexes were analyzed by Western Blotting.
Dbait-protein complexes were analyzed on 6% polyacrylamide gels (29∶1).
Next, immune complexes were analyzed by immunoblotting.
Complexes were analyzed by Superose 6 10/300 chromatography.
Immune complexes were analyzed by western blotting with specific antibodies.
