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Non-hydrolysed films, however, offer improved retention of bound complexes.
Such insoluble proteoliposomes cannot even be applied to receptor-binding experiments for subsequent affinity purification of the bound complexes.
[TLR9-DNA] represents the concentration of the bound complexes.
After extensive washing with CB300, bound complexes were eluted with FLAG-peptide and separated by SDS-PAGE.
Since the structures of the last two complexes are not available experimentally, we first modelled the bound complexes.
HRP-conjugated anti-IgM was used to reveal bound complexes using and OD was recorded at OD450.
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The ligand-bound complexes of Census protein structures were obtained from canSAR3D, a component of canSAR that includes weekly updated structural data and compound ligand annotations.
Importantly, in cBAF-perturbed cancers, ncBAF complexes maintain gene expression at retained CTCF-promoter sites and function in a manner distinct from fusion oncoprotein-bound complexes.
Makowska-Grzyska, M. et al. Bacillus anthracis inosine 5′-monophosphate dehydrogenase in action: the first bacterial series of structures of phosphate ion-, substrand-, and product-bound complexes.
Clearly, both Keap1-CDB and ENC1-Myc were present in CBD-bound complexes (Fig. 4A).
Tight control on the expression of membrane-bound complexes is probably essential in many eukaryotic systems.
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