Sentence examples similar to bound column from inspiring English sources

To address this question, we reapplied the flow through to a clean metal chelate column, and observed that no additional PfBCCP-79 bound the column (data not shown).

Low values indicate that high MW aggregates might have bound to column material or parts of HPLC system; nd, not detected; ws, weak signal.

In both the HEPES/NaCl and BisTris/KCl conditions, the β-lactamase eluted in the flow-through while the majority of impurities bound the column.

SERPINA3K was purified by passing through the His-Bind column (G.E).

Escherichia coli BL21 DE3 pLysS cells transformed with the pRSET A/TCTPs or pRSET A/mutated TCTPs were overexpressed and purified using a Ni2+-charged His-Bind column according to manufacturer's protocol (Novagen).

The supernatants were applied to a Ni-NTA His-bind column for purification.

Bind column in accordance with the manufacturer's instruction (Millipore, USA).

The supernatants were transferred to a Ni-NTA His-bind column for purification.

The released DNAs and the DNA from the internal controls were purified with GenElute Binding Column G.

After the construct comprising rv1985c was transformed into Escherichia coli Rosetta (DE3), His-tagged Rv1985c protein was expressed by 1.0 mM Isopropyl-β-D-galactopyranoside (Sigma) and purified under native conditions by His-Bind Column (Novagen) according to manufacturer's recommendation.

Reconstituted samples were equilibrated to 5 mM tricine with 2 mM KCl (pH 7.6) by passage through a PD-10 column and bound to a column of DEAE Trisacryl (Sigma-Aldrich, St . Louis MO, USA).