Sentence examples for bound cells were washed from inspiring English sources

Beads bound cells were washed six times with fresh, sterile PBS.

The column was washed, removed from the magnet and bound cells were washed out.

CD44high bead-bound cells were washed three times with PBS plus 2% FCS and incubated with DNaseI to release the cells from the beads via DNA linker cleavage.

The antibody-bound cells were washed three times in PBS for 5 minutes each, and fluorescent secondary antibodies were added for 1 hour at 37°C.

Antibody-bound cells were washed and resuspended at 1 × 10 cells per mL in FACS buffer and run on a FACSCalibur flow cytometer (BD Biosciences).

Antibody-bound cells were washed and resuspended at 1 × 106 cells/ml in FB and run on a FACSCalibur flow cytometer.

To eliminate surface-bound HRP-Tf, cells were washed 3 times for 10 min with ice-cold isotonic citrate buffer (150 mM NaCl and 20 mM sodium citrate (pH 5.0).

The cells were washed and the bound protein was detected as described above using anti-human-allophycocyanin and flow cytometry.

To distinguish cell-surface-bound complexes from internalized complexes, the transfected cells were washed with a heparin salt that should compete with DNA-cell-surface interactions removing the fluorescent label (see Supporting Information, Figure S5).

The cells were washed twice with PBS and the bound labeled cargoes were allowed to internalize for different time points by incubating the cells at 37°C.

After incubation the cells were washed thrice with PBS to remove extracellular bound Cd.