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Dynabead-bound cells were discarded, a quarter of the original number of Dynabeads was added to the cells, and a second round of negative selection was performed as above.
Non-adherent cells were discarded after 60 min.
When FCS was used for supplementation and non-adherent cells were discarded, both media were comparable.
The collected samples were centrifuged at 10,000 rpm for 5 min and cells were discarded.
Cells were discarded when the series resistance values were over 20 MΩ.
The plate was incubated at 37°C for 2 h, and the non-adherent cells were discarded.
Nonadherent cells were discarded.
After 3d, non-adherent (granulocyte-rich) cells were discarded.
Non-adherent cells were discarded after 24 h culturing.
Non-adherent cells were discarded after cultivation for 48 h.
Non-detached cells were discarded.
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