Sentence examples for bound beads were washed from inspiring English sources

Protein bound beads were washed extensively with PBS +1% Triton X-100 and subsequently PBS +0.1% Triton X-100, and stored O/N at −20°C in PBS +75% glycerol +0.1% Triton-X100.

Briefly, the supernatant of homogenized cells was incubated with glutathione-agarose beads at room temperature for 30 min. The protein bound beads were washed several times before incubating with PreScission™ Protease (GE Healthcare, Piscataway, NJ) at 4°C for 4 hrs, which eluted InlA with a mass of 50 kDa at a final yield of 5 mg/L of culture.

To the remainder of the sample was added 10 µg of GST-PBD beads, and following 60 min incubation at 4°C the GST-PBD-Rac-GTP bound beads were washed 3 times with lysis buffer supplemented with protease inhibitor cocktail.

Unbound antibody was removed and the antibody bound beads were washed with PBS-Tween before addition of the cell lysate.

The protein bound beads were washed two times with 15 ml buffer E, followed by 15 ml of buffer F (buffer A + 0.5% Triton X-100, 250 m m KCl and 2 m urea) at 4°C.

Bound beads were washed sequentially with 0.1%and0.05%5% Tx-100 followed by water.

Precipitate-bound beads were washed 3-times in wash buffer.

The protein-bound beads were washed three times with lysis buffer, and then eluted with SDS sample buffer.

The supernatants were discarded; arginase-bound beads were washed with 2 × 1 mL of IMAC lysis buffer by mixing and centrifugation, and the supernatant was discarded.

Protein-bound beads were washed thoroughly with wash buffer and equilibrated into cleavage buffer (50 mM Tris pH 7.4, 250 mM NaCl, and 1 mM DTT).

The fusion protein-bound beads were washed three times with 500 μl of TEN buffer (20 mM Tris HCl [pH 7.4], 0.1 mM EDTA, 100 mM NaCl).