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Bound beads were loaded onto a disposable column (as above) and rinsed with the remaining unbound fraction.
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Beads with displacements less than 5 nm (limitation of resolution detection) and loosely bound beads were not selected for analysis.
Finally, enriched beads were loaded onto picotitre plates for sequencing.
Approximately 800,000 DNA-carrying beads along with enzyme and packing beads were loaded onto a PicoTitrePlate device.
After bead enrichment, beads were loaded onto Ion 316 chips and sequenced using an Ion Torrent PGM.
According to the manufacturer's recommendations for amplicon sequencing, 250,000 DNA beads were loaded for each pool mixed with an appropriate volume of packing beads and enzyme beads.
Beads with amplified libraries were loaded onto GS FLX Titanium PicoTiterPlate.
Tissue samples (153 416 mg) were loaded into bead beating tubes (Percellys Steel-Kit) preloaded with three steel beads.
Proteins obtained from the MOLT-3 cells were loaded onto the four types of Sepharose 6B beads and mixed overnight at 4°C, and the proteins that bound to the four types of Sepharose 6B beads were then analyzed by SDS-PAGE.
Each vial of DC Bead (2 ml of beads) should be loaded with 50 75 mg doxorubicin (loading dose, 25 37.5 mg doxorubicin/ml of beads).
Proteins bound to the beads were eluted with SDS-loading buffer at 99 °C for 5 minutes and then loaded for western blot, and 2 μg of whole cell lysates were loaded as input.
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