Sentence examples for bound beads were boiled from inspiring English sources

Bound beads were boiled in reducing SDS PAGE sample buffer.

Following immunoprecipitation experiments, antigen-antibody bound Protein G beads were boiled in SDS-loading buffer; solubilized proteins were run on 8% or 4-124-12%dient SDS-PAGE.

After extensive washes, the proteins that bound to the beads were boiled in SDS-loading buffer, resolved with a 4-20% SDS-PAGE gel and visualized by coomassie blue staining.

Blank sepharose beads were used as a negative control, and samples of beads were boiled in SDS PAGE sample buffer to check for bound protein.

Finally, beads were boiled with loading buffer and analyzed on a 12% SDS-PAGE gel.

The magnetic beads were boiled in 2× SDS loading buffer, and separated on polyacrylamide gels (10%).

The beads were boiled in Laemmli buffer and the proteins resolved by SDS-PAGE (10% gel).

Beads were boiled in SDS buffer to release active Rac1.

Beads were boiled for another 10 min, centrifuged, and the supernatant was collected.

Sample buffer was added, and the beads were boiled and subjected to western blot analysis.

Beads were boiled in sample buffer for 5 min, and then subjected to SDS-PAGE.