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They share a common structure of two 4 domains linked by a long helix, with the metal bound at the interface between the domains.
In chain A, the FAD is noncovalently bound at the interface of a Rossman-type domain and the substrate-binding domain.
The thiamine-dependent enzymes are all oligomeric and are organized as homodimers, homotetramers, or heterotetramers, with the ThDP cofactor bound at the interface between subunits.
In this respect it is worth reminding that PSI-LHCI supercomplex is peculiar because it contains a pigment complement, constituted by "Gap" and "Linker" Chls, which are bound at the interface between the core and the antenna moiety [ 41, 46].
The helicase binds to DNA substrate via the DRD domain and promotes the rotation between two ATPase domains to form a closed XPB-DNA complex in which ATP is bound at the interface of the two ATPase domains.
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Many biochemical studies support the view that the juxtaposed G- and T-segments bind at the interface of the B'/A' DNA binding and cleavage core and the ATPase domains [1].
It follows that these Arf-GEFs are predicted in vivo targets for the fungal metabolite Brefeldin A (BFA), an established inhibitor of the secretory pathway which binds at the interface between the Sec7 domain and Arf-GDP in vitro [17], [18].
Two FMN molecules are bound at the dimeric interface.
(A) Cholesterol close to LxxL (cyan) and CRAC domains Y413 (green) and Y570 (purple); (B) cholesterol bound at the dimer interface; (C) cholesterol bound to CRAC Y570 in the second ABCG2 subunit.
The shear bound at the contact interface between the concrete slab and the steel beam is simulated by using the contact modeling and considering the adhesion, the friction and the local compression at all the contact zones.
We also present a crystal structure of the IN core domain in complex with sucrose which is bound at the dimer interface in a region that has previously been reported to bind integrase inhibitors.
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