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Ferri-siderophore complexes are bound at the cell surface by specific outer membrane receptors and transported into the periplasm, where a periplasmic binding protein delivers the complex to an inner membrane transporter [5].
To strip-off lipoplexes bound at the cell surface, cells were treated with 1 mL glycine (0.2 M, pH = 2.8) for 30 min at 4°C and rinsed in ice cold PBS before FACS analysis [26], [27].
Plg bound at the cell surface may then be activated to pln by cell-surface or possibly stromal cell-derived [ 43- 46] uPA.
Following the transfection, the cells were washed three times with heparin (200 μL; 2 mg/mL) for 1 h to remove the DNA complexes bound at the cell surface.
A processing pathway in which the full-length mature enzyme is bound at the cell surface and cleaved to generate both identified truncated forms by membrane-type 4-MMP (MT4-MMP, also termed MMP-17) has been proposed [ 85].
Since the amount of Ga or Cr in TC137 (with YxeB) was the same as the amount in TC111 (no YxeB), the Ga and Cr levels of the wild-type strains represent imported metal-siderophore, not metal-siderophore bound at the cell surface by YxeB.
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Although we find no detectable binding of STAT5 to Eλ3−1 in pro-B cells, it is bound at the Irf4 promoter in pro-B cells but not in pre-B cells.
Consideration of changes in RNApol2 binding in hypoxia revealed that for the majority of hypoxia-inducible genes, RNApol2 was already bound at the promoter in normoxic cells.
Nonetheless c-Jun in these cells was bound at the c-Jun promoter (Fig. 3C).
One pathway provides a rapid, nongenomic pathway initiated by membrane bound ERs at the cell surface [1] [3].
Alternatively, secreted IL-6 can bind to the soluble IL-6 receptor (sIL-6R), which then binds gp130 at the cell surface and initiates intracellular signaling.
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CEO of Professional Science Editing for Scientists @ prosciediting.com