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The bound antibodes were eluted with elution buffer.
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After incubation with the sera, the beads were washed, and bound antibodies were eluted.
After extensive washing, bound materials were eluted with Laemmli buffer and analysed by immunoblotting.
The column was washed extensively and bound proteins were eluted by reduction of the biotin conjugate.
After extensive washing with CB300, bound complexes were eluted with FLAG-peptide and separated by SDS-PAGE.
Spirochetes incubated in EDTA-treated NHS were washed extensively and bound proteins were eluted.
Unbound proteins were removed and bound proteins were eluted competitively with a concentrated RMS-P3/RR peptide solution.
After 10 cycles of washing with 1x TBS, bound phages were eluted using 0.2 M glycine, pH 2.2.
Subsequently, bound phosphopeptides were eluted under acidic conditions.
Specific bound phages were eluted, amplified and used for next panning as previously described [ 14].
Following several washes, bound nucleotides were eluted and quantified as above.
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