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As the cholesterol bound and unbound forms of NPC1 NTD) crystallized in the same spacegroup, the conformation of apo-NPC1 NTD) apo-NPC1 NTDobservedinus study may have been the result ourcrystal previous
Our measurements lack the spectral resolution to differentiate between the bound and unbound forms of NADH or NADPH, therefore the NAD(P H component represents all NADH and NADPH fluorescence [13].
Since COPI subunits are soluble proteins that transiently cycle between vesicle membrane bound and unbound forms as a function of interactions with Arf proteins [29], [30], the observed differential abundance of COPI subunits in the two midgut microsomal fractions suggests that they could be associated with RER whorls.
Figure 5A shows a comparison of the success rates between the predictions for bound and unbound forms as query proteins.
We retrieved 352 protein complex structures from a standard protein-protein docking benchmark set (ZLAB Benchmark 4.0) [ 23], which contained bound and unbound forms of the protein structures.
This result differs from the asymptotically decreasing distribution observed in our previous study (Najmanovich et al., 2000) in which the most common case was that of binding sites with no rearrangements between the bound and unbound forms.
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Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments suggested that fast exchange (< 10 μs) between the bound and unbound form of Nup153FGPxFG gives rise to the increased R2 rates around the interaction sites, which makes it possible to estimate a residue-specific Kd,individual for each position in Nup153FGPxFG with Importinβ from the population weighted R2 measurements.
The nature and the strength of the stacking interaction between these BODIPYs and DNA has been addressed through a detailed study of the photophysical properties of the bound form and unbound form of the dyes.
Active p38 (ATP bound and unbound) can reversibly bind to ATF2 or MK2 to form complexes p38-ATF2 or p38-MK2, respectively.
Addition of SAM to the ΔCTD protein resulted in a mixture of SAM bound and unbound states, allowing for accurate g-value assignment of each cluster form; the C96A/C100A/C103A protein containing only the accessory C-terminal [4Fe 4S] cluster was shown to not bind SAM, indicating that despite its site-differentiated nature it cannot substitute for the N-terminal cluster.
As we were only measuring total (bound and unbound) β-catenin in this study, a change in the ratio of bound and unbound β-catenin was not determined.
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bound and free forms
bound and unbound fractions
bound and soluble forms
bound and unbound guests
bound and unbound structures
bound and unbound proteins
bound and unbound states
bound and unbound orientations
bound and unbound volumes
bound and unbound populations
bound and unbound fluorophores
bound and unbound sequences
bound and unbound ligands
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