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The indole-containing acceptor substrate is bound adjacent to the prenyl donor.
To test this hypothesis, a fluorescent nanocircuit reported the position of the myosin lever arm only when the myosin was bound adjacent to the troponin, or in controls, only when the myosin was bound distant from the troponin.
In addition to a magnesium ion, which is essential for catalysis, a potassium ion bound adjacent to the triphosphate moiety of ATP in a rat mitochondrial protein kinase, BCK (branched-chain α-ketoacid dehydrogenase kinase), has been shown to be indispensable for nucleotide binding and hydrolysis.
In the crystal structure, the substrate is well-ordered across the active site, and the azide anion is observed bound adjacent to Ala 32.
Therefore, although no actual substrate molecule is present in this structure, RebC appears to assume a conformation as though a substrate molecule were bound; i.e., this reduced-RebC structure seems to represent a stage in the catalytic cycle where substrate is bound adjacent to reduced flavin.
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The discoligamentous complex (DLC) consists of intervertebral discs and spinal ligaments that bind adjacent vertebrae to each other and guide segmental motion and contribute to the intrinsic stability of the spine by limiting excessive motion.
This specific example now states that Dali and CTCF "might independently bind adjacent sequence, or compete for binding to the same region".
Consequently, Dali and CTCF may be non-interacting molecular subunits of a larger ribonucleoprotein complex, or alternatively they might independently bind adjacent sequence, or compete for binding to the same region.
We and others have shown that these shed adhesion molecules bind adjacent ECs via their respective ligands, exert a direct angiogenic effect on local ECs, and facilitate angiogenesis [ 5].
In conjunction with a previously reported Tat-cyclin T1 structure (Tahirov et al., 2010), Alber and colleagues show that AFF4 and Tat bind adjacent to each other on cyclin T1.
In some cases, these motifs may recruit proteins that bind adjacent to Rbf1 to provide specialized responses, similar to the way that modulatory proteins in mammals bind near E2F sites to functionally differentiate subclasses of these promoters (Jin et al. 2006; Freedman et al. 2009).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com