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"I knew how bound I was, restricted.
Homeward bound, I was dropped at Odessa airport and noticed a crowd outside the terminal.
And, spring after spring, I watch as girls with corn-silk hair soar off campus in open cars, bound, I imagine, for supernal débutante parties, nymph dances amid rolling pastures beyond the last commuter stop, in some mystic arch-suburb that is the real Pennsylvania.
By Elizabeth Minkel June 30, 2011 Months back, when I heard that Alan Hollinghurst's new novel was finally being printed and bound, I priced out flights to England — and then I looked up shipping rates on amazon.co.uk, which were fairly reasonable and, I grudgingly admitted, a better way to get a book across the ocean.
We now bound I 11 and I 13.
Forever bound, I am, but I came to this conclusion by way of layers of depth and complexity.
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All cell lines specifically bound I-IL-4, as binding was competed by 200-fold excess of unlabeled IL-4 (Fig 1B).
Radiochemical purity assessment of radiolabelled antibody by ITLC prior to injection confirmed >98.6% bound I- (1.4% free) and >99.3% bound In-CHX-A″-DTPA.
I-CXCL11 binding was robust among all mutants, except for the D112N mutant, which bound I-CXCL11 at detectable but significantly reduced levels compared with WT CXCR3.
Afterward, duplicate 200 μL aliquots were transferred to centrifuge tubes and centrifuged at 14000 rpm for 5 min. The bound I-labeled ligand was counted.
In control cells, about 42% of the originally bound I-GH was degraded and secreted as free iodide within 2 h (Fig. 2C, right panel).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com