Sentence examples for bound Abs were visualized from inspiring English sources

For detection, HRP-labeled goanti-mouseuse or anti-rabbit antibodies (Bio-Rad) in TBST were used and bound Abs were visualized by chemiluminescence (ECL-system, Amersham Corp).

The membrane was washed and the bound Abs was visualized by developing with NBT/BCIP as chromogens.

After washing, bound WT1 IgG Ab was visualized for each well using 100  μL of BCIP-NBT kit (Nacalai Tesque, Kyoto, Japan).

After a final wash, bound antibodies were visualized by SuperSignal West Pico chemiluminescent substrate kit (Pierce).

Following further washing, bound antibodies were visualized as above.

Subsequently, bound antibodies were visualized using the anti-rabbit Envision Plus System (K4011, Dako, Glostrup, Denmark).

After final washing, bound secondary antibodies were visualized using enhanced chemiluminescence (Super Signal ECL, Pierce, and IL).

Following five washes with 0.1% (vol/vol) Tween 20 in PBS and five washes with PBS, bound serum antibodies were visualized with 50 μl of isotype and class-specific sheep anti-human immunoglobulin, anti-IgG, or anti-IgM antisera, each labelled with horseradish peroxidase (Silenus Laboratories, Hawthorn, Australia) and diluted to 1 500 in 1% (vol/vol) AB serum and incubated for 1 hour at 37°C.

The bound Abs and MAbs were visualized by Alexa-633 or 555-conjugated anti-mouse IgG, or Alexa-555-conjugated anti-rabbit IgG (Molecular Probes).

Binding profiles were visualized with the Integrated Genome Browser (IGB) browser [87].

Binding antibodies were visualized by using ECL and ECL Plus kits (GE Healthcare).