Sentence examples for bound Abs were detected from inspiring English sources

Briefly, ELISA plates were coated with most antigens at 1 µg/ml, blocked with casein in PBS, incubated with antibodies, and bound Abs were detected with the appropriate peroxidase-conjugated second antibodies.

The bound Abs were detected using (as required): PE-conjugated (Fab' 2 fragments of goat anti-mouse IgG (H+L) or Fluorescein (FITC -conjugated (FITC -conjugated oFab' 2 anti-human IgG (H+L).

After washing, bound Abs were detected using AP-conjugated swine anti-rabbit IgG (diluted 1∶1000 in TBST/0.5% BSA; Dako, Glostrup, Denmark) or AP-conjugated rabbit anti-mouse IgG+IgM (diluted 1∶5000 in TBST/0.5% BSA; Jackson ImmunoResearch, West Grove, PA, USA).

Bound Abs were detected as described above.

Bound Abs were detected by enhanced chemiluminescence (ECL, Millipore, USA).

The bound Abs were detected using Immobilon Western Chemiluminescent HRP substrate (Millipore).

After six washes, bound Ab were detected with biotinylated anti-mouse IgG1 (BD8-2, BD) or anti-mouse (5.7, BD) mAb or with biotin-SP-conjugated anti-mouse IgG F ab′ 2 (Jackson Immunoresearch).

Antigen-bound Abs were detected using HRP-conjugated goantinti mouse IgH+L (P.A.R.I.S).

All biotinylated ABs were detected with Streptavidin-APC (Biosciencesces).

HSV-2 specific Abs were detected by HerpeSelect 2 ELISA IgG (Focus Diagnostic, Cypress, CA).

Furthermore, PGRN Abs were detected in patients with spondyloarthritis.