Sentence examples for bottom was probed with from inspiring English sources
The blot was cut in half, and the top was probed with polyclonal antiserum specific for TRAP as a loading control (top panel), and the bottom was probed with mAb 3D11 specific for the CSP repeat region (lower panel).
The bottom part was probed with α- Pgk1; the top part was first probed with α-P-CREB, stripped for 30 min at 60° with Tris-SDS buffer [62.5 mM Tris HCl (pH 6.8), 2% SDS, 100 mM β-Mercaptoethanol], and then probed with α- Msn2.
The top piece (>75kD) was developed for CFTR protein (150 to 180 kD) using 3G11 rat monoclonal antibody [ 30], the middle piece (between 75kD and 35kD) was probed for α-tubulin (approximately 55kD) as an internal control (DM1A antibody, GeneTex), and the bottom piece (<35kD) was probed with TTC35 antibody (sc-166011, Santa Cruz Biotechnology).
Bottom, α-tubulin loaded in each lane was probed with anti-α-tubulin Ab. 15 μg of whole brain membrane proteins were loaded in each lane.
To demonstrate that equal amounts of plexinA ectodomain were used for the IP experiment, an aliquot of the input was probed with anti-myc (blot at bottom).
Odd numbered gradient fractions (indicated above the blots) were probed with anti-ySnu114 (top) and anti-yPrp3 (bottom) antibodies.
Flag immunoprecipitated K64R mutants or wt variants were probed with H3K64ac antibody (top), and anti-HA antibody as loading control (bottom).
