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Height of the center bobbins above the bottom was measured using a bottom contact sensor developed at the AFSC.
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This study demonstrated that arable farming provides a range of ES which can be measured using field experiments based on ecological principles by incorporating a 'bottom-up' approach.
Therefore, roughness of the bottom channel surface was measured using Atomic Force Microscopy (AFM) scans on the surfaces.
The random migration in four directions – top, bottom, left and right – was measured using an inverted Leitz microscope.
Quantification of phenotypes, including number of ICVs, number of ectopic SIV sprouts and length of the subintestinal basket (the distance between the vPCV and the bottom end of the SIV), was measured using ImageJ (National Institutes of Health).
Cells were resuspended in PBS and transferred to a clear bottom 96-well plate, and GFP fluorescence was measured using a plate reader (485 nm excitation and 515 nm emission).
The deflection of the actuator was measured using electric strain gages mounted on the top and bottom surfaces of the actuator.
Exploratory activity was measured using a Y-maze apparatus (arm length: 40 cm, arm bottom width: 3 cm, arm upper width: 10 cm, height of wall: 12 cm).
Reaction temperature was measured using a thermocouple inserted in a stainless-steel thermocouple well locating at the bottom center of the catalyst bed.
CAP was measured using a double sucrose gap chamber [30] which was specially made with a glass coverslip bottom to facilitate simultaneous CARS imaging of the same sample.
MPI was measured using ImageJ.
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