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However, the bottom phase is almost dilute brine.
The bottom phase was extracted and washed with hexane three times.
Both biomass and recombinant protein partitioned to the bottom phase during extractive bioconversion.
Insoluble substrate and immobilized enzyme were biased in bottom phase, while product was partitioned in top phase.
By adjusting uneven partition of substrate and products between the top phase and bottom phase, their inhibition effect is removed.
It was found that α-la and Gmp partitioned to the top phase rich in PEG, whereas β-lg partitioned to the bottom phase rich in salt.
Substrate, Penicillin G enters into bottom phase, and it is catalyzed into 6-APA and phenylacetate acid, then the products enter into top phase.
The bottom phase (approximately 3.5 mL), composed of acidified water and part of the ethanol originally present in the gasoline sample, containing the extracted analytes was analyzed.
The fibrinolytic enzyme was shown to preferentially partition to the bottom phase with a partition coefficient (K) ranging from 0.2 to 0.7.
In such conditions β-lg preferentially concentrates in the bottom phase, while the top phase exclusively contains the proteins α-la and Gmp.
Systems based on dextran and polyethylene glycol (PEG) proved to be optimal for partitioning the enzyme to the bottom phase which is rich in dextran T500.
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CEO of Professional Science Editing for Scientists @ prosciediting.com