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When the cells approximately covered all the bottom of the tissue culture flask, Hanks buffered salt solution (HBSS, pH7.4) was used to wash the cells three times.
Afterwards the cells were mechanically removed from the bottom of the tissue culture dishes in 1 mL ice cold PBS each.
Three different sites of heart walls from the top, middle, and bottom of the tissue were analyzed: subepicardium, myocardium, and subendocardium.
In YE medium very few dead cells were detected, which were scattered and attached to the bottom of the tissue culture plate).
Similar patterns of microscopic reentry can be found also by initiating the propagation from the bottom of the tissue; see Figure 8.
Tumor content in the TCGA samples is determined by a pathologist who evaluates a slice from the top and a slice from the bottom of the tissue block used for RNA isolation to quantify the percent tumor content.
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For measurement of granulation tissue thickness, the thickest distance from top to bottom of the granulation tissue was used.
There are microcalcifications at the bottom of the breast tissue in this case– 9(l)).
c) Meat juice (500 μL), collected from the bottom of the muscle tissue storage bottle.
At the bottom of the mammographic tissue appears an isolated microcalcification– 9(p)).
At 1 month, group B showed intense positive results for ChM-I in the bottom of the repair tissue.
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