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Exact(10)
To avoid attachment on the bottom of the plates, the aspirates were carefully mixed after each medium change.
After incubation, the magnet was removed and the cells were detached from the bottom of the plates, forming a contiguous cell sheet.
Readings were carried out from the bottom of the plates with no shaking and recorded every 30 min. Plates were sealed to prevent evaporation.
Briefly, hemocytes exposed to UV were detached from the bottom of the plates with a cell scraper (Falcon) and loaded into cytospin cuvettes (Cytospin 4, Thermo Scientific).
Under these conditions, cells attached to the bottom of the plates, where they were left to differentiate for 20 additional days, changing the medium as required.
The cells were allowed to adhere to the bottom of the plates for 24 h in complete media and then exposed to drug or vehicle in fresh media for the indicated periods of time.
Similar(50)
Spread the dressing over the bottom of the plate and place the grilled leeks on top.
A slightly gelatinized base made with freshly squeezed pea juice covered the bottom of the plate.
The mixed meat plancha, simple and satisfying, was amplified by a single line of mint pesto at the bottom of the plate.
The eggplant with shrimp is deep-fried, but there's little feeling of grease; the sauce is not poured over the vegetables but coats the bottom of the plate.
Present theory exactly satisfies stress boundary conditions on the top and the bottom of the plate.
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CEO of Professional Science Editing for Scientists @ prosciediting.com