Sentence examples for bottom of the filter from inspiring English sources

Cells on the bottom of the filter were stained with crystal-violet and counted with an inverted microscope.

Moreover, coculturing prevented the transport of NP from the epithelial compartment to the endothelial layer on the bottom of the filter insert.

It was then held upside down, and a filter paper was placed at the bottom of the filter.

The remaining cells (migrated to the bottom of the filter) were imaged and counted using a light microscope (Leica).

Cells migrating to the bottom of the filter were stained with DAPI Vector Labss, Burlingame, CA), photographed with an epifluorescence microscope, and counted.

Cells on top of the filter were removed, while cells on the bottom of the filter were fixed with PFA and stained with rhodamine phalloidin and DAPI.

After 3 hours, the cells on the bottom of the filters were labeled with the fluorescent dye calcein AM (4 µg/ml).

The migrating cells at the bottom of the filters were counted in four fields per filter in three independent experiments.

Cells (1×10) were seeded on the bottom of the filters and were incubated at 37°C with 5% CO2 for 5 hrs to adhere.

SVEC4-10 cells in DMEM containing 1% FCS were added to the upper chamber for 4.5 h and then migrated cells on the bottom of the filters fixed, stained and counted at ×160 magnification in three random fields per filter.

After 24 h, cells that have either trans-migrated (in control chambers) or invaded and reached the bottom of the filters were scored at five random fields of observation, and averaged for the replicate wells.