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Install plugs in bottom of filter, pump, and basket.
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Go into your basement and you'll find the filter on the bottom of the filter casing.
After setting the label, hit the blue "Create filter" button at the bottom of the filter creation window.
Invading cells on the bottom surface of filter were fixed in methanol and stained with Giemsa.
After removal of the remaining cells from the upper surface of the filter, migrated cells at the bottom of the filter were fixed with 3.7% formaldehyde in PBS and stained with 0.1% crystal violet.
Bleed the pressure from your Aqua-Pure unit by pressing down on the red button (pressure relief button) on the top of the filter housing Take your filter wrench and slide it up from the bottom of the filter housing towards the head.
It was then held upside down, and a filter paper was placed at the bottom of the filter.
Transwell 8 μm pore filters were underlaid (coated on the bottom of the filter) with 50 μL control or tamoxifen mammary ECM at a concentration of 75 μg/mL and dried overnight.
Cells on top of the filter were removed, while cells on the bottom of the filter were fixed with PFA and stained with rhodamine phalloidin and DAPI.
The aeration was positioned at the bottom of the filter and ensured a continuous friction of air bubbles against the sides of the filter screen, which resulted in an efficient reduction of filter-mesh clogging (see Fig. 4.4.4.).
Samples were washed by filling the filter portion with 4 mL of buffer (ensuring resuspension of the sample from the bottom of the filter) and then centrifuged again for 75 minutes to ensure the dead volume was reached.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com