Sentence examples for bottom of each well from inspiring English sources
A porous polymeric membrane sealed at the bottom of each well accounts for the sensor support.
It is to be noted that, before plating cells, a resized sterile plastic coverslip (Thermanox) was placed on the bottom of each well.
In this system, cells adhere to the bottom of each well, covering the surface of the sensor that monitors cells by measuring their cell index that is translated from the electronic readout.
To prevent evaporation, 0.5 ml phosphate-buffered saline was placed in the bottom of each well.
MS1 cells were plated into 24-well plates that contained glass coverslips at the bottom of each well.
The measurement was performed using OxoPlate® (PreSens, Germany), 96-well plates which contain an immobilized oxygen sensor at the bottom of each well.
Briefly, gridded coverslips (with 175 µm grids, Eppendorf AG, Germany) were placed on the bottom of each well in 24-well plates.
50 µl of base agar matrix (Cell Biolabs; CytoSelect™ Cell, Colorimetric kit) was added in the bottom of each well of a 96-well plate.
Cell invasion assays were performed by seeding cells in Boyden chamber (BD Bioscience) coated with matrigel in serum-free medium with or without IGF-1 (50 ng/ml) in the bottom of each wells for 18 hours.
Plates were incubated for 12 to 16 h at room temperature to conveniently fix the anti-histidine antibodies to the bottom surface of each well.
Media in the bottom chamber of each well were supplemented with 0.2% FBS and 100 ng/ml Wnt5a to trigger chemotactic migration.
