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Recordings were made from parathyroid cells that had been allowed to settle on the bottom of a chamber placed on the stage of an inverted microscope (Olympus IMT-2, Tokyo, Japan).
The ganglion, dissected together with the sympathetic trunk, was desheathed and pinned to the bottom of a chamber mounted on the stage of a compound microscope; individual neurons were identified at a magnification of ×500 by using diffraction interference optics.
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To ensure efficient quantum capture, the cells were placed on the bottom of a recording chamber and images were recorded after 10∼20 s when fluorescence intensity became stable.
The eyecup was flat mounted, sclera side down, directly on the bottom of a recording chamber and was superfused by oxygenated (95% O2/5% CO2) Ames medium (Sigma-Aldrich, St . Louis MO) at a fixed rate (5 ml/min) at room temperature between 22 24°C.
Immediately after dispersal, cells were plated at the bottom of a recording chamber for amphotericin perforated patch-clamp experiments.
Using tissue glue (Histoacryl B, Braun, Germany), the dissected head was then glued in the middle of a glass slide at the bottom of a flow chamber.
Briefly, healthy Z. mays L. root apices (5-6 mm long) were cut, carefully washed with deionized water, and placed individually at the bottom of a measuring chamber containing an electrophysiological solution (10 mM CaCl2, pH 6.5).
Rings of thoracic aorta (2 3 mm long) were cut open along the longitudinal axis and pinned down to the bottom of an experimental chamber, endothelial side upward.
Furthermore we observe for the given impedances and thicknesses of the setup employing a capillary at the bottom of the chamber a non-vanishing acoustic force of about 10% of the maximum force.
A twin-plane ECT sensor with eight electrodes in each plane is mounted in the bottom of a glass fluidisation chamber.
Field stimulation was performed via two platinum wires (0.5 cm separation) placed at the bottom of a customized perfusion chamber.
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