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Shale samples (about 5 × 5 × 5 mm3) were first dried at 110 °C for 1 week, then glued to the bottom of a cell, which was then filled with solid Wood's metal pieces.
A total of 3 mL of solution was poured into the bottom of a cell culture dish.
A two-photon section at the very top or bottom of a cell might have an excitation depth that only partially penetrates the cell and the sampled membrane area would be consequently smaller.
For primary tumour cell culture, effusions (20 500 ml) were centrifuged, cell pellets washed twice in PBS (Biochrom, Berlin, Germany),resuspended in RPMI 1640 medium supplemented as described and then seeded on the bottom of a cell culture flask.
Under these conditions, no effect of soluble ephrin-A1 was observed–(d)), which might implicate that clustering of the ligand on the cell surface of neurons or at the bottom of a cell culture dish is required for efficient stimulation of the EphA receptors.
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Using this technique, we were able to separate the probes from the top and bottom layer of a cell bilayer.
To demonstrate the effectiveness of this technique we measured the viscoelastic properties of the top and bottom layers of a cell bilayer.
The difference in GFP-PHPLCδ labeling of comet tips and of the plasma membrane was even more striking on images of the bottom plane of a cell showing intracellular comets that encountered the plasma membrane and evaginated it into filopodia-like structures, akin to those generated by Listeria-driven comets.
We found that cells in the monolayer exhibited similar MSD to cells in the bottom layer of a cell bilayer, indicating that the addition of cells to the top of a monolayer does not substantially alter the monolayer's properties (Fig. 6 ).
On the first day, the cells were seeded as a monolayer on a local part on the bottom of a tilted cell culture dish (100 mm × 20 mm, CELLSTAR®, Greiner Bio-One) and incubated with 2-mL medium for 24 h in order to avoid cell migration.
The immobile NAPL mass is placed at the bottom of a flow cell filled with porous media with water flowing horizontally on top.
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