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One day prior to transfection approximately 10 cells were seeded in 15 mm dishes with thin glass bottoms (Mat-Tek).
Stick the bottom most mat to the bed or surface for which the user is using the product on.
For imaging experiments, cells were seeded on No 1.5 glass bottom dishes (Mat-Tek Corp., Ashland, MA) prior to transfection.
Specimens were mounted in glass bottom dishes (Mat-Tek, Ashland, MA).
Cells were grown in Dulbecco's modified Eagle's medium, supplemented with 10 U/ml penicillin, 50 μg/ml streptomycin, and 10%% fetal calf serum, on a glass-bottom dish (Mat-tek).
Slow decay and mechanical disturbances break off waterlogged bits from the bottom of the mat.
The Sierra Portable Light mat, bottom, is made by weavers in Mexico using L.E.D.'s; it extends the workday for people living off the grid in rural areas.
The plant roots grow through the bottom of the mat, adding microbes that will eventually yield clean water and provide food for fish.
35 mm glass bottom culture dishes (Mat Tek Corporation) were used for cell culture and two photon analyses.
Cells were grown on glass bottom micro-plates (Mat Tek), and transfected with EEA1-EGFR plasmid for 16 h.
Move the foam roller to the bottom of your mat.
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