Sentence examples for bottom dish from inspiring English sources

Dissociated cells were suspended in MEM containing 5% hiHS and 5% FCS, and plated onto coverglass bottom dish pre-coated with poly-d-lysine.

Cells grown on a collagen I-coated glass bottom dish supplemented with phenol red-free medium, were exposed to 2 or 8 Gy X-ray 48 h after siRNA transfection.

For imaging 100 µl of the GUVs were mixed with 100 µl of isotonic buffer (90 mM Tris pH 8, 20 mM MgCl2, 40 mM Imidazole, 565 mM NaCl, 3 mM βME) in a BSA coated 4-chamber glass bottom dish (In Vitro Scientific).

To trace mBSA in live cells, 1.5 × 103 BV-2 cells were seeded onto a cover-glass bottom dish.

For immunostaining of microtubules, cells were plated into a 35 mm-glass bottom dish (MatTek Corp).

Cells were plated on a 35-mm glass bottom dish (Iwaki).

Alternately, isolated cells were placed on a 35-mm glass-bottom dish with 2% methylcellulose and covered with BCDAT agar medium separated with a sheet of cellophane.

H4-GFP-TSCs were seeded onto a 35-mm glass-bottom dish and cultured in TS medium without FGF4/heparin to induce differentiation.

PC3 cells were seeded in a 4-chamber glass-bottom dish (MatTek, USA) and 24 hours later transfected with 0.5 μg of either GFP-EN2 or GFP (control) plasmids as described above.

For ex vivo brain culture, the brain explants were mounted on a glass-bottom dish (35 mm MatTek petri dish, 20 mm microwell with 0.16/0.19 mm coverglass) in a fibrinogen clot prepared by adding thrombin (bovine thrombin, Sigma) to fibrinogen (bovine fibrinogen, Calbiochem) as previously described64.

Hela cells were cultured in a homemade 35-mm glass-bottom dish in DMEM (Sigma) containing 10% fetal bovine serum (BioWest).