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A nutrient gradient was established by filling the bottom compartment with FBS rich medium (30 vol%).
Large, black Hermes golf accessory bag including set of lock and key, and extra strap in bottom compartment, presented in cream colored drawstring bag.
BM-MSCs were then plated in the bottom compartment of the Transwell at a density of 2.5×105 cells/well with indirect contaction with H9C2 cells.
The problem of rupture of the emulsion droplet due to pressure created by direct dispersion of gas at the bottom of conventional RDC extraction system was addressed by adding an impeller at the bottom compartment of RDC.
Improved design of BTD successfully reduced the chance for pressure meter test (PMT) membrane breakage, allowed larger expansion of borehole jack test (BJT), improved the waterproof of the bottom compartment and made easy for the whole equipment setup.
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(b) Summary of key Hox genes expressed in neuronal (top) and mesodermal (bottom) compartments along the anterior posterior axis in fish and tetrapods43,46,47,49,51.
Non-melanized C. sphaerospermum was added to the top compartments and melanized C. sphaerospermum was added to the bottom compartments of each plate.
The stimuli were added in both upper and bottom compartments of ALI culture system to provide the access to both apical and basolateral receptors of ECs.
The assembled chamber was incubated at 37°C in a humidified atmosphere of air and 5% CO2 for 90 min. Compounds under investigation were added in equal concentrations to both the top and bottom compartments of the migration chamber.
In all Transwell experiments, the volumes of media in the top and bottom compartments were 0.5 mL and 1.5 mL, respectively.
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