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Bottles were closed with rubber stoppers and cannulas were used for gas outlet and sampling, thus minimizing oxygen transfer.
These bottles were closed with a rubber stopper through which a hollow needle was fed to guarantee a constant oxygen supply.
All bottles were closed with a viton stopper and aluminium cap in the anaerobic glove box, and the headspace was changed to N2/CO2 (80%20%%).
The bottles were closed with viton stoppers (Maag Technik, Dübendorf, Switzerland) and aluminum crimp caps, and the head space was replaced by N2 gas (140 kPa).
The bottles were closed with a viton stopper with crimp cap and incubated horizontally shaken (110 rpm) in the dark at 30°C.
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Every bottle was closed with inner seal white caps to ensure its safety, prior to consuming.
The bottles were closed under water to prevent contamination by air.
Red wines that may be aged in the bottle for many years are closed with corks 2 inches (5 centimetres) long or longer.
By the way, the Dry River was one of just three bottles among our 20 to be closed with a cork.
After complete overflow of the bottle and the tin and continuous flushing for at least several minutes to remove atmospheric air from the sample bottle, the tube was pulled out and the bottle was closed under water in the tin.
The serum bottles were flushed with argon gas for 5 min to create an anaerobic condition before being closed with rubber stoppers, capped with aluminum caps, and sterilized in an autoclave at 110 °C for 28 min.
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