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It was stored in 500 ml glass bottles and kept in a refrigerator until use.
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For each shrimp pond flocculation, three replicates of floc sample (20 mL) were collected and kept in a plastic bottle and then the samples were fixed in 10%% formaldehyde.
Alternatively a tincture is made with a piece of the vine and kept in a snake bottle.
For analysis of nutrients, surface water samples were collected in clean polyethylene bottles and kept in an ice box and transported immediately to the laboratory.
After filtration, the extract was concentrated to 1/3 initial volume and then centrifuged at 4000 rpm for 15 min and kept in a dark bottle at 22 °C for further uses.
To minimize the spillage of liquids during the sucrose test, bottles are filled in advance and kept in an up-side-down position for at least 12 hours prior to testing.
The dried extract of each plant part was collected in labeled airtight small bottles and kept in deep freezer until used.
For nutrient analysis, pre-rinsed polyethylene bottles were filled with depth integrated samples and kept in an icebox for later analysis (usually within 24 72 h).
Decant into bottles, seal tightly and keep in a cool dark place for a further 3 months before drinking.
The resultant extract was concentrated to dryness under reduced pressure below 40°C in rotary evaporator and stored at 4°C in a tightly capped brown bottle and kept in refrigerator for the study.
Bottle into warm, sterilised jars and keep in a cool, dark place for up to a year.
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