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The BOTTLENECK test provided evidence that this S. brachyodon population has recently experienced severe reductions in the effective population size, i.e. a bottleneck.
Using Garza and Williamson's bottleneck test, M ratios ranged from 0.67 to 0.89 for neutral loci across all populations and years (Tables 3 and S15).
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Taken together, highly reduced levels of genetic variation in introduced L. leucozonium populations along with significant bottleneck tests and lack of population structure suggest that this bee experienced a single and severe founder event during its introduction to North America.
Results of bottleneck tests using bottleneck and M-ratio using 90% single-step mutations.
The BOTTLENECK tests [ 58] provided no evidence for recent bottlenecks in neither LIL, HAL nor MOS.
BOTTLENECK tests for testing the departure from mutation-drift equilibrium were based on heterozygosity excess or deficiency.
Furthermore, a dramatic decline in effective population size was not detected in our microsatellite bottleneck tests (Table S4).
Bottleneck tests based on the heterozygosity excess method did not indicate severe preceding bottlenecks in any of the temporal groups or in the modern-day reference sample.
Population bottleneck tests suggested early N e decline (prior to 1920; Table 4) and also for historical wolves collected after 1959 (Table 1).
Cornuet and Luikart (1996) suggest a minimum sample size of 20 diploid individuals to achieve a reasonably high degree of power in bottleneck tests.
Although the reliability of various bottleneck tests to detect the occurrence and timing of genetic bottlenecks has been questioned, particularly the M-ratio test (Peery et al. 2012), we used multiple approaches and found concordant results across these approaches.
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