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Thus, the current bottleneck in understanding RNA mediated interaction is the correct identification of genes that may be targeted by the numerous smRNAs in the cell.
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Recently significant progress has been made in understanding the bottlenecks in recombinant protein expression using plant cells, including advances in plant genetic engineering for efficient transgene expression and minimizing proteolytic degradation or loss of functionality of the product in cell culture medium.
Further dissecting the biological factors favoring transmission through the maternal-infant bottleneck, particularly characterizing newly transmitted or founder viruses, will be critical in understanding the molecular mechanism of MTCT and in guiding novel intervention approaches.
While there is great expectation and an understandable emphasis on translational research in many quarters, we believe that the challenges in understanding fundamental epigenetics and chromatin processes remain bottlenecks in achieving these goals.
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We mention that effector activation in complex with Ras on the membrane is structurally unresolved, a major bottleneck in our understanding of Ras biology.
Despite increasing attention and research efforts on this topic, a theoretical bottleneck and a technological bottleneck continue to impede further advancement in understanding, modeling, and managing preferential flow.
One bottleneck in the development of hMSC-derived therapies is our incomplete understanding of the mechanisms governing hMSC differentiation.
However, our understanding of viral diversity and virus host interactions remains a major bottleneck in the development of predictive ecosystem models and unifying eco-evolutionary theories.
A lack of understanding of the molecular basis of this inhibition has been a major bottleneck in the development of next generation of drugs essential for targeting the mycolic acid component of mycobacteria.
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