After incubating for 120 h, the content of an entire bottle was collected and centrifuged.
90% Of post-consumed PET bottles were collected by informal collectors (i.e., scavengers and itinerant waste buyers).
Four of the bottles were collected from the reef surface (∼10 m depth), two bottles were collected 25 cm above the benthos, and two bottles were collected 500 cm above the benthos.
The bottles were collected between May and July 2002.
The bottles were collected between May and July 2002, which corresponds to the end of fall and the beginning of the dry winter season in Sao Paulo.
5, 6 A total of 30,210 pairs of blood culture bottles were collected during the study period (10,208, 12,574 and 7,428 in 2009, 2010 and 2011, respectively).
The residual alimentary and cosmetic oil bottles were collected at the time of the next delivery in order to check the adherence of the participants to the allocated treatment.
Spent grain (30 tonnes a week) is fed to a local dairy herd, yeast slurry (fifty tonnes a week) is fed to pigs, waste beer (30 litres a week) is drunk by dairy cows, and bottles are collected and recycled to make drinking glasses.
Cans, glass, paper and plastic bottles are collected on a weekly basis.
"What we really need are system changes to reduce the volume of throwaway plastic packaging and make sure plastic drinks bottles are collected and separated effectively," said Edge. "An enzyme alone can't clean up the complex and widespread legacy of plastic pollution that we have already created".
