Sentence examples for both wt and mutation from inspiring English sources

For human CYP21A2, the membrane anchor and basic region were replaced with MAKKTSSKGK from CYP2C3, and both WT and mutation V359A were expressed in E. coli BL21 DE3) cells and purified to homogeneity using Ni-NTA affinity resin (Qiagen, Valencia, CA) after solubilization with 1% 3-[ 3-cholamidopropyl dimethylammonio]-1-propanesulfonate as described for bovine CYP21A2.

Using a reverse primer, we verified that both wt and point mutation neu transcripts co-existed in 83923 cells (Fig. 3b).

For the splice-site prediction programs (Splice Site Finder, MaxEntScan, NNSplice), wild-type score (wt) and mutation score (mut) were compared using the following cutoffs.

There was a borderline significant difference (P = 0.051) in baseline Ki67 between PIK3CA WT and mutation samples, with patients with the mutation having lower levels of proliferation (Table  2).

Ki67 levels were suppressed by median values of 69%and77%7% in patients with WT and mutation GSs, respectively, but this difference was not statistically significant (Table  2 and Additional file 6: Figure S1).

Since HSD10 WT and mutations are localized to mitochondria and patients with MHBDD show signs of mitochondrial dysfunction, we analysed mitochondrial morphology by EM in fibroblasts derived from patients compared with control fibroblasts (Fig 1K and Supporting Information Fig 3).

In contrast, we found the co-presence of either Kir4.1 or Kir5.1 with Kir2.1 in the protein eluates derived from both WT- and K346T-expressing cells, although the mutation did not affect the possible interactions between these subunits (Supplementary Material, Fig. S3).

Finally, we compared the rates of mutation for each gene between WT and KO, mutation rate (fold change) greater than 2 were considered as possible genetic variation effects.

The performance improvement process is comprised of four sequential steps of k-means clustering, peptide selection (PS), weight training (WT) and matrix mutation (MaM).

However, whereas the mtDNA mutation loads were consistently higher in mutator cells than in WT cells, no specific mutations were present at high enough frequencies to account for the different characteristics between WT and mutator cells.

The resulting fragments containing the wild type (Wt) and mutated XIAP-BIR2 with the linker region and XIAP-BIR3 were sequenced and cloned into the XhoI-EcoRI site of pDsRed-Monomer-C1 (BD Biosciences) to generate pDsRed-XIAP-BIR2-Wt, pDsRed-XIAP-BIR2-3M containing D148A/E219R/H223V mutations, pDsRed-XIAP-BIR3-Wt, and pDsRed-XIAP-BIR3-1M containing E314S mutation, respectively.