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Exact(5)
PAX7, however, was weakly dorsalized in both wildtype and talpid 3+/− embryos.
PITX2 expression was consistently identified in the left lateral plate mesoderm in both wildtype and talpid embryos (Fig. 6e,f) and was, therefore, normal.
CEP164 localized correctly at the mother centriole and not at the sister centriole, in both wildtype and talpid 3 cells of the neuroepithelium and fibroblasts.
At E10, both wildtype and talpid female embryos exhibit regression of the right gonad (talpid n = 8 as demonstrated through dissection, histology (GR; Fig. 1e,f), and PITX2 expression (talpid n = 3; not shown).
LEFTY1 expression was observed from 4HH in the node of both wildtype and talpid embryos and in the developing primitive streak until 6 7HH, when expression was briefly restricted to the left of the node (not shown).
Similar(55)
At 12HH in wildtype and talpid chicken embryos, however, heart looping is normal (Fig. 1c,d, n > 100).
(C′ ) Quantification of the percentage of feather buds with correct, abnormal or no polarity in wildtype and talpid (D, E ) SEM of the basilar papilla in wildtype (D ) and talpid 3 (E ) chickens.
Subdistal appendages were identified in approximately 40% of wildtype and talpid 3 centrioles (wt n = 35, talpid 3 n = 48, Figure 4D,E,G), whereas distal appendages were noted in 28% of wildtype centrioles and only 6% of talpid 3 centrioles.
Having identified normal expression of SHH and the presence of cilia in the early talpid embryo, we examined expression of downstream laterality markers in wildtype and talpid embryos.
Expression of SHH in wildtype and talpid embryos is seen throughout the rostral notochord in a bilaterally symmetrical manner between 3 and 4HH (Fig. 5a,b).
From 4+HH to 8HH SHH expression became normally lateralized to the left-hand side of Hensen's node in wildtype and talpid embryos (arrows, Fig. 5c f).
Related(20)
both wildtype and nuc-1
both wildtype and ctf7
both wildtype and heterozygous
both wildtype and dominant
both wildtype and anc1
both wildtype and Egfr
both wildtype and homozygous
both wildtype and fopC
both wildtype and pcd
both wildtype and gEnull
both wildtype and transgenic
both wildtype and pad2-1
both wildtype and transfected
both wildtype and anc1Δ
both wildtype and Smad2
both wildtype and mHttex1
both wildtype and cIAP-1
both wildtype and OPN-deficient
both wildtype and Shroom3
both talpid and talpid
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