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Most of the cytosolic long-chain acyl-CoAs are used for β-oxidation in mitochondria, and approximately 80%% of acetyl-CoA in the heart of a model of T2DM, db/db mouse, was found to be fatty acid-derived both when perfused with low glucose/low fatty acid buffer and when perfused with high glucose/high fatty acid buffer [ 97].
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In conclusion, hepatocytes in the BAL bioreactor showed preservation of important metabolic functions, when perfused with homologous toxic plasma.
COS-7 cells co-expressing a low-affinity FLIPPi and a Na+/Pi co-transporter exhibited FRET changes when perfused with 100 μM Pi, demonstrating concentrative Pi uptake by PiT2.
CONCLUSION: Lungs from swine expressing human decay accelerating factor and human CD59 functioned better than nontransgenic swine lungs when perfused with human plasma.
These results suggest that complement activation is involved in producing acute pulmonary xenograft dysfunction and demonstrate that lungs from swine expressing human decay accelerating factor and human CD59 are protected against pulmonary injury when perfused with human plasma.
In striking contrast to the in vivo microdialysis data, cultured astrocytes preloaded with [3H]taurine failed to show any increase in taurine release levels when perfused with the same low [NaCl] isoosmotic medium (Fig 2a).
They also, with the notable exception of HEK cells expressing RyR2-G1885E/G1886S, readevelopedloped SOICR activity when perfused with increasing [Ca2+]o.
In contrast, slices from cKO mice showed profound changes in excitability when perfused with 100 nM kainate (Fig. 7d f).
More than 90% of FLZ was bound to the tubing when perfused with ringer's solution containing FLZ.
Permeabilized myotubes generate force when perfused with calcium solutions, with a similar calcium sensitivity of force generation compared to mature skeletal muscle fibers [ 21].
The ONIX microfluidic perfusion system (CellASIC Corp., USA) was used to analyze the dynamic responses of A. fumigatus cells when perfused with caspofungin-supplemented medium.
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