However, no interaction between IRE1 and TNFR1 was detected when immunoprecipitating IRE1.
Interestingly, we confirmed interaction between RIPK1 and IRE1 when immunoprecipitating either IRE1 or RIPK1.
Conversely Nrz was pulled-down when immunoprecipitating Flag-Bcl-wav showing that these two proteins were able to form a heterocomplex (Fig. 1C).
As expected, this was specific for the long isoform of dATRX, since dHP-1a-FLAG specifically enriched for the long isoform of dATRX when immunoprecipitated from cell extracts (Figure 5, asterisk, upper panel).
As shown previously, when immunoprecipitated with a GFP antibody, full length NEDD1 and the CTD were able to co-precipitate γ-tubulin, but the construct that terminated at residue 571 did not (Figure 4B).
When HERG was immunoprecipitated the amount of KCNE2 that co-precipitated was strikingly increased whereas the amount of KCNE1 that associated with HERG was not significantly altered (Figure 4B, middle and right panels).
When α-actinin was immunoprecipitated, the amount of co-immunoprecipitated GluN1 was higher from ICAM-5 KO mouse brain as compared to that of WT mice (Fig. 3A,B).
Using antiserum directed against virus-coded proteins, it was found that phosphorylation of the heavy chain of the antibody occurred when immunoprecipitates containing src protein were incubated with [γ-32P]ATP.
Furthermore, the specificity of the reaction was demonstrated when the human anti-Su sera immunoprecipitated the in vitro translated Dicer, but the human control serum did not.
When samples immunoprecipitated from the media were boiled in loading buffer prior to electrophoresis, the 40 kDa band collapsed into the 20 kDa species suggesting the existence of a dimer (Figure 5B).
