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The genome-wide transcription response of the CON and FMP strains, when both were grown in triplicates, resulted in 985 genes with differential expression (padjusted<0.01).
E. coli strains, both were grown in Luria-Bertani (LB) medium.
Mean vessel diameters in the IL-12-expressing tumours were significantly smaller than in the parental tumours when both were grown in wild-type mice.
This was corroborated by the later flowering time of the parents under 16-hr light than under 12-hr light, when both were grown in a controlled environment.
The A2780 A2780wtt) ovarian carcinoma cancer cell line was purchased from the European Collection of Cell Cultures (ECACC, Wiltshire, UK), and the MCF-7 (MCF-7wt) breast cancer cell line from the American Tissue Culture Collection (ATCC, Middlesex, UK), and both were grown in RPMI supplemented with 10% FBS, 2 m M L-glutamine, 1% non-essential amino acids and 0.075% sodium bicarbonate.
Loss of function of ZRT3 was also observed to cause an increase in the Cd sensitivity of this strain compared to the control (BY4741) when both were grown in liquid culture (Additional file 2: Figure S1), validating the elevated Cd observed in this strain.
Similar(54)
Both are grown in Yirgacheffe, Ethiopia, but processed differently — processing being the way a coffee bean is removed from the coffee cherry.
Both accessions of both species were grown in a fixed media volume, so differences in root characteristics (e.g., rooting depth) could not be expressed.
Both microorganisms were grown in lab culture media in petri dishes at 20 °C until cells reached the stationary phase.
Both strains were grown in hydrolysates [30] and culture samples were periodically withdrawn.
Both strains were grown in YPD with shaking at 30°C (A) or 39°C (B).
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