Exact(1)
Thus, space constraint on 1D-culture is likely to account for the observed reduction of both velocity and directionality.
Similar(59)
Likewise, leukocyte interstitial migration upon intrascrotal injection of PAF displayed a diffuse character as shown by low levels of leukocyte straight-line velocity and directionality on both vessel sides.
Data was imported into Microsoft excel and average velocity and directionality calculated.
Monocytes, however, did not move toward MCP-1, at least at 60 65 min after microinjection, as indicated by lower levels of curve-line and straight-line migration velocity and directionality (Fig. 8B, C, D).
By contrast, the effect of microinjection was stronger at the level of straight-line velocity and directionality, parameters that demonstrate how far and how directly leukocytes move toward the stimuli within the time period analyzed.
Interestingly, in contrast to microinjection, leukocyte interstitial migration was rather random upon intrascrotal injection of PAF (Fig. 4Ba d) or MIP-1α (Fig. 4Ca d) as shown by significantly lower straight-line migration velocity and directionality (Fig. 5B, C).
Moreover, upon microinjection of chemoattractants or bacteria, leukocyte straight-line velocity and directionality were several times higher on the vessel side ipsilateral to microinjection as compared to those on the contralateral side.
Interestingly, straight-line velocity and directionality of monocytes were slightly increased after 70 min and 80 min after microinjection of MCP-1 as compared to those after 60 min (Fig. 8C, D).
The target-oriented character of interstitial leukocyte migration after microinjection of chemoattractants or bacteria is underlined by the finding that the elevation of straight-line migration velocity and directionality was several times higher than the increase in curve-line velocity (Fig. 5A, B, C).
On the ipsilateral side, leukocyte interstitial migration was target-oriented towards the site of microinjection (Fig. 4Aa d) and characterized by significantly increased curve-/straight-line migration velocity and directionality as compared to leukocytes migrating in saline-treated cremaster muscles or to leukocytes migrating on the contralateral vessel side (Fig. 5A, B, C).
The quantitative analysis of leukocyte interstitial migration demonstrated that perivenular microinjection of MCP-1 induced target-oriented migration of neutrophils within the interstitial tissue as shown by increased curve-line and straight-line migration velocity and directionality (Fig. 8A D) compared to those after microinjection of saline (Fig. 5A, B, C).
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