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Both variants were able to adhere to, and invade, YAMC cells.
As expected, both variants were able to dimerise as shown by crosslinking with BS (Supplementary Fig. S2a).
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Noteworthy was that even though both mucoid and non-mucoid variants were able nodulate M. flocculosa, those induced by the non-mucoid variants were ineffective in fixing nitrogen and inferior in promoting root growth (Additional file 6: Figure S2, Additional file 7: Table S5).
Neither the extralong MnP6 nor the two mutated variants were able to oxidize DMP, as both VP1 and LiP-H8 did, suggesting that the engineered sites were unable to properly bind this phenolic substrate.
It has been found that six out of seven frequent variants were able to cause the invalidation of famous neutralizing MAb 2F5.
All variants were able to activate allergen-specific T cells in PBMC, as well as Art v 1-specific T cell lines and clones.
However, these variants were able to suppress aggregation during arginine kinase (AK) refolding as efficient as wild-type rhCyPA, especially, variant Q63A had even more efficiency to suppress aggregation and improve reactivation yields of AK.
Polyclonal antibodies produced against the simplified variants were able to recognize the parent peptide, but not the opposite simplified forms, in strict agreement with the model of independent surfaces of recognition.
In a bacteria-delivered model for enzyme prodrug therapy, the engineered FRase I variants were able to kill human colon carcinoma (HCT-116) cells at significantly lower CB1954 concentrations than wild type FRase I or E. coli NfsB.
Both 20 kDa and 40 kDa star-shaped PEG variants were investigated, and all four PEG-peptide variants were able to undergo a sol gel phase transition when mixed with the linear C7 protein at constant physiological conditions due to noncovalent hetero-dimerization between the C and P domains.
The [44AANA47]-CCL5 and Met-CCL5 variants were able to significantly reduce the swelling 24 h post challenge.
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CEO of Professional Science Editing for Scientists @ prosciediting.com