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Indirect immunofluorescence was performed on both unfixed and fixed spirochetes.
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As controls, we analyzed both unfixed hESCs and fixed hESCs [10].
In this work, we carried out SVGs with unfixed and fixed samples.
These results using unfixed and fixed chromatin indicated that not only the linker DNA regions, which flank the nucleosome core, but also the DNA surrounding histone core was more easily digested by MNase after day 6 of differentiation in dTSCs than in TSCs.
Taken together, we conclude that the Tubb3-mGFP transgenic mouse line allows the identification of newborn neurons by their intrinsic mGFP fluorescence at the systemic, tissue and cellular level in unfixed and fixed tissue.
To understand the effects of glutaraldehyde fixative on adhesion force, we firstly performed two groups that included unfixed cells and fixed cells respectively (which did not incubate in culture medium), and the group of fixed cells was set as control I.
The enrichment analysis between unfixed COG groups and fixed COG groups (Additional file 1: Table S1) in each COG sub-category shows that the sub-category of 'secondary metabolites biosynthesis, transport and catabolism' contains significantly more unfixed COGs (P-value < 0.05).
To validate our automated hybridization method for FFPE material we compared CGH profiles from unfixed and formalin fixed paraffin embedded SKBR3 cells.
The evaluations included an overall estimation of the entire sections from both the chemically unfixed and fixed specimens of each muscle.
(d) Fraction of intra-TAD PETs by Trac-looping in unfixed and fixed cells.
(c) Fraction of intra-chromosomal PETs by Trac-looping in unfixed and fixed cells.
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