Because single protein-coding or rRNA transcript-tags from a putative microorganism were frequently encountered, our confidence in taxonomic assignment increased by employing bioinformatics methods to classify organisms based on both types of transcripts.
Both types of transcripts use canonical splice sites for all exons coming from both genes.
lncRNAs associated with ribosomes (lncRNA_ribo) showed higher coding scores than those not associated with ribosomes (lncRNA_noribo), even when we did not use the ribosome profiling information and compared the longest ORF in both types of transcripts.
A similar mechanism might explain the lower expression observed for both types of transcripts in the LP cultivar, where the lack of oleuropein could be due to differences in the regulation of enzymes involved in its biosynthesis rather than in its degradation.
Both types of chimeric transcripts have an open reading frame using the start codon from PPARG exon 1 and lack the STOP codon of the PPARG gene situated in exon 6, the only exon missing in the chimeric transcripts.
Both types of chimeric transcripts contain all but the last exon of the PPARG gene and all but the first exon of the TSEN2 gene, which are normally the sites of termination and initiation of transcription, respectively.
We attempted to study both types of effects, including transcript levels and allele frequencies of candidate genes involved in sulfur assimilation and methionine biosynthesis as well as seed storage protein transcript and protein levels.
Another functional group found in both types of wounds contains transcripts encoding elements of cytoskeleton (group 2 in tongue and group 5 in skin) (Table 8, additional file 8 and 9).
As both types of endoderm express many transcripts in common (Foxa2, Gata4, Gata6, Sox17, Hnf4α, Afp, Ttr,.), one should take into account the minimal differences in transcript and protein expression between these two types of endoderm when investigating in vitro differentiation of pluripotent stem cells towards hepatic endoderm.
