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The rice husk with highest C/N ratio of 22.7, showed maximum spore production in both types of medium.
Although the BP prediction model may lose resolution in distinguishing small changes in the case depth around 0.07 mm, the average prediction error of case depth in both types of medium carbon steel rods is lower than 5%.
Both types of medium were supplemented with 10% fetal bovine serum.
It is worth pointing out that in both types of medium the concentration of glucose in the extracellular environment is undetectable soon after reaching the stationary phase.
In this study, the housekeeping gene Gapdh was used as a positive control for mononucleated cells in both types of medium, i.e., proliferation and differentiation medium.
The results obtained for the partial purification of PGA present in crude extracts obtained from cells grown in both types of medium (complex and defined) are shown in Table 2.
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Solid media contained 2% agar for both types of mediums.
Both types of culture medium (LB and PCAT) gave consistent results.
The ASCs successfully adhered to the scaffolds, and cell density increased with prolonged duration of incubation time in both types of culture medium.
Generally, combining different types of medium, using both solid and liquid media, increases the effectiveness of culture, perhaps because of a preference of the bacterium for one type of medium over another or simply from the increased sensitivity obtained by culturing a large volume of specimen.
Prior to exposure to differentiation medium, both types of cells showed similar multilineage phenotype (CD44+/MAP2+/GFAP+/Vimentin+/Beta III-tubulin+/Fibronectin+) and were positive for SOX-2 and Nestin.
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